OHSU to be one of the first to install new high-resolution mass spectrometer

Thermo Scientific Orbitrap Fusion
Thermo Scientific Orbitrap Fusion. Image: Business Wire

This July, OHSU’s Proteomics Shared Resource will be adding a high-resolution mass spectrometer called a Thermo Scientific Orbitrap Fusion. OHSU will be one of the first universities in the world to install this new flagship proteomics platform by Thermo.

The Orbitrap Fusion will allow OHSU researchers to incorporate the latest technologies in proteomics including tandem mass tags, iTRAQ, and SILAC multiplexing of complex protein samples, as well as improve their detection and quantitation of protein modifications, such as phosphorylation, acetylation, and ubiquitination. The system will also have an automated robot to speed H/D exchange experiments to support protein structure studies.

The new instrument’s resolution is so high that it can detect mass defects caused by a neutron’s association with different nuclei. Being able to detect this mass defect will be a key technology that allows multiplexing of more samples into single LC/MS runs. This is a major game changer in proteomics, because it will allow an experiment to have many biological replicates without significantly increasing the analysis cost.

The purchase of the instrument was made possible both by an NIH S10 Shared Equipment Grant and support from the OHSU University Shared Resources Program. More information will be made available this summer when the instrument becomes operational. The Proteomics Shared Resource will also be hosting an open house so that researches can come see the new instrument and learn more about its capabilities.