CRISPR/Cas9 gene editing comes to OHSU

OHSU scientists: you have a new technology available to you–with special pilot pricing available. Several new methods for genome engineering have been introduced in recent years, the most successful of which is the clustered regulatory interspaced short palindromic repeats (CRISPR)/Cas9 system. The OHSU Transgenic Mouse Models Core (TMM) has initiated several preliminary CRISPR pilot projects based in part on a recent campus-wide interest survey. Recently, OHSU’s first F1 mice genetically modified using CRISPR mice were produced by the TMM (Lev Fedorov, Ph.D., Director), in collaboration with the lab of Peter Barr-Gillespie, Ph.D. At least four different knockout alleles of the Espnl gene were generated; these required less than six weeks from initiation of the project to F0 mice. All alleles transmitted to the F1 generation. In addition, the TMM has currently initiated pilot CRISPR knock-in projects to optimize methodologies for this approach and is prepared to extend pilot program pricing to other OHSU investigators for a limited time. For additional details, visit the TMM website.